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MAISRC Webinars

A Novel Technology for eDNA Collection and Concentration
 
October 28, 2020 at 1 PM CDT
 
 
Presentation Summary: Assessing species presence and distribution is one of the most critical steps in the study of biodiversity. This is also necessary to monitor invasive species that can adversely impact the ecosystem and the economy. Early detection of aquatic invasive species is crucial in designing strategies to combat them. A common method used for this purpose is to identify the environmental DNA (eDNA) or genetic material shed by these species into the aquatic environment. However, current methods used for sampling and separation of eDNA faces challenges in obtaining results that correlate with traditional netting data. Although several studies have explored different types of filters to capture eDNA, the recovery yield is still limited and all commercially available filters only target tissue/cell eDNA and are unable to recover free eDNA. Aquatic environments contain both tissue, cell and free eDNA. Recovering the free eDNA could potentially provide some missing pieces of information on aquatic invasive species. In this study, we report a novel technology for the collection and concentration of aquatic eDNA including free eDNA using a nanosorbent. Preliminary results show that these novel filters can collect up to 100 % of free eDNA and concentrate it up to 300 times. Ongoing research aims at improving eDNA recovery and developing a filter housing system for field deployment. Register here.
 
 
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The AIS Detectors program at the University of Minnesota is jointly supported by the Minnesota Aquatic Invasive Species Research Center and University of Minnesota Extension. We create volunteer, citizen science, and educational opportunities focusing on aquatic invasive species issues. Our webinar series launched in May 2019 and features webinars on a variety of aquatic invasive species topics. The webinars are always free to attend and closed captioned recordings can be found out our YouTube channel. You can subscribe to our channel here.